Journal: International Journal of Oral Science

Article Title: Single-cell transcriptomics identifies PDGFRA + progenitors orchestrating angiogenesis and periodontal tissue regeneration

doi: 10.1038/s41368-025-00384-6

Figure Lengend Snippet: Reciprocal interaction of PDGFRA + dental MSCs with ECs potentially contributes to angiogenesis and osteogenesis. a Interaction strength heatmap of dental follicle cell clusters. b Interaction strength heatmap of dental papilla cell clusters. c Dot plot showing significant ligand–receptor pairs related to angiogenesis between DFSCs and SCAP with ECs. d Western blot analysis of PDGFBB expression in DFSCs and HUVECs. e ELISA quantification of VEGFA concentrations in the conditioned medium from cultured DFSCs with and without PDGFBB treatment. Data were presented as mean ± SD. n = 3 per group. * P < 0.05; ** P < 0.01; ns not significant ( P > 0.05)

Article Snippet: All experiments involving DFSCs treated with PDGFBB were conducted by supplementing the culture medium with PDGFBB (HY-P7055, MCE, China) at a concentration of 100 ng/mL when the cell confluence reached 40%-50%.

Techniques: Western Blot, Expressing, Enzyme-linked Immunosorbent Assay, Cell Culture

Journal: International Journal of Oral Science

Article Title: Single-cell transcriptomics identifies PDGFRA + progenitors orchestrating angiogenesis and periodontal tissue regeneration

doi: 10.1038/s41368-025-00384-6

Figure Lengend Snippet: Endothelial PDGFBB treatment improves the functionality of PDGFRA + DFSCs. a Representative images of colonies formed by DFSCs (scale bar = 250 μm) and EdU staining images showing DFSC proliferation (scale bar = 50 μm). b Alizarin red S staining images showing DFSC osteogenesis. Scale bar = 100 μm. c Quantification of colony diameter in CFU assay. d Quantification of EdU + cells percent (%). e Quantification of fold change of mineralized area. f Quantification of the fold change of ALP activity. Data were presented as mean ± SD. n = 3 per group. * P < 0.05; ** P < 0.01; *** P < 0.001; ns not significant ( P > 0.05)

Article Snippet: All experiments involving DFSCs treated with PDGFBB were conducted by supplementing the culture medium with PDGFBB (HY-P7055, MCE, China) at a concentration of 100 ng/mL when the cell confluence reached 40%-50%.

Techniques: Staining, Colony-forming Unit Assay, Activity Assay

Journal: International Journal of Oral Science

Article Title: Single-cell transcriptomics identifies PDGFRA + progenitors orchestrating angiogenesis and periodontal tissue regeneration

doi: 10.1038/s41368-025-00384-6

Figure Lengend Snippet: Paracrine promotion of CD31 + EMCN + vessel formation by PDGFRA + DFSCs is safeguarded by endothelial PDGFBB. a , b Representative images showing tube formation of HUVECs treated by conditioned medium of DFSCs (scale bar = 250 μm) and IF staining images showing CD31 + EMCN + vessel formation of HUVECs treated by conditioned medium of DFSCs (scale bar = 200 μm). c , e Quantification of tube formation of HUVECs. d , f Quantification of CD31 + EMCN + vessel percent (%). DFSCs were pretreated with or without PDGFBB. Data were presented as mean ± SD. n = 3 per group. * P < 0.05; ** P < 0.01; ns not significant ( P > 0.05)

Article Snippet: All experiments involving DFSCs treated with PDGFBB were conducted by supplementing the culture medium with PDGFBB (HY-P7055, MCE, China) at a concentration of 100 ng/mL when the cell confluence reached 40%-50%.

Techniques: Staining